Caspase-12 Antibody #35965
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Product Name
- WB
Inquiry Info. # 35965
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Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 42, 55 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Caspase-12 Antibody recognizes endogenous levels of total full-length caspase-12 protein (55 kDa) and its cleaved product (42 kDa). Bands of unknown origin were detected at 12, 25, and 70 kDa.
Species Reactivity:
Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys163 of mouse caspase-12 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER stress-induced apoptosis, such as high calcium concentration, low oxygen, and low glucose levels (1,2). One of the mechanisms for caspase-12 activation is related to calpain-mediated cleavage at T132 and K158, both of which are located at the amino-terminal region of caspase-12 (2,3). Caspase-12 also has a putative caspase cleavage site located at the carboxy-terminal region of the protein (3). In cortical neurons, caspase-12 is involved at least partially in the amyloid-beta neurotoxicity process (1).
Limited Uses
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