Caspase-12 (E9T3W) Rabbit mAb #58208

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Supporting Data

REACTIVITY	M
SENSITIVITY	Endogenous
MW (kDa)	55, 38, 28
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Caspase-12 (E9T3W) Rabbit mAb recognizes endogenous levels of total Caspase-12 protein. This antibody detects the large subunit of Caspase-12 associated with activation. Bands of unknown identity were observed at 42 and 30 kDa. These may represent additional caspase-12 isoforms.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to a region within the large subunit of mouse Caspase-12 protein.

Background

Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER stress-induced apoptosis, such as high calcium concentration, low oxygen, and low glucose levels (1,2). One of the mechanisms for caspase-12 activation is related to calpain-mediated cleavage at T132 and K158, both of which are located at the amino-terminal region of caspase-12 (2,3). Caspase-12 also has a putative caspase cleavage site located at the carboxy-terminal region of the protein (3). In cortical neurons, caspase-12 is involved at least partially in the amyloid-beta neurotoxicity process (1).

Database Links

UniProt ID: O08736

Entrez-Gene Id: 12364

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For Research Use Only. Not For Use In Diagnostic Procedures.

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