Caspase-8 (F5K9P) Rabbit mAb (BSA and Azide Free) #46272
- WB
- IHC
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 10, 57 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
For standard formulation of this product see product #8873
Storage
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
In addition to functioning as a key initiator caspase for extrinsic apoptosis, more recent studies have identified caspase-8 as a regulator of inflammatory necrotic cell death pathways such as necroptosis and pyroptosis (4,5). Activation of caspase-8 leads to cleavage of RIPK1 and RIPK3 to inhibit necroptosis (6,7). As a result, caspase-8 deficiency in mice, which is embryonic lethal, can be rescued by deletion of the necroptosis proteins RIPK3 or MLKL (8-11). Additionally, in some circumstances, caspase-8 is recruited to the inflammasome to trigger pyroptosis (12,13). Studies have also found that expression of an enzymatically inactive form of caspase-8 (C362S) causes embryonic lethality by inducing necroptosis and pyroptosis (14).
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- Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.
- Mandal, R. et al. (2020) Biochim Biophys Acta Rev Cancer 1873, 188357.
- Han, J.H. et al. (2021) Int J Mol Sci 22, 3318. doi: 10.3390/ijms22073318.
- Newton, K. et al. (2019) Nature 574, 428-431.
- Feng, S. et al. (2007) Cell Signal 19, 2056-67.
- Varfolomeev, E.E. et al. (1998) Immunity 9, 267-76.
- Kaiser, W.J. et al. (2011) Nature 471, 368-72.
- Oberst, A. et al. (2011) Nature 471, 363-7.
- Alvarez-Diaz, S. et al. (2016) Immunity 45, 513-526.
- Man, S.M. et al. (2013) J Immunol 191, 5239-46.
- Antonopoulos, C. et al. (2015) J Biol Chem 290, 20167-84.
- Fritsch, M. et al. (2019) Nature 575, 683-687.
Limited Uses
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