Render Target: STATIC
Render Timestamp: 2024-12-20T11:30:41.698Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-11-14 23:10:27.914
Product last modified at: 2024-11-15T09:00:10.589Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Caspase-9 (C9) Mouse mAb (BSA and Azide Free) #10068

Filter:
  • WB
  • ELISA

    Supporting Data

    REACTIVITY H M R Hm Mk
    SENSITIVITY Endogenous
    MW (kDa) 47/37/35 (H). 49/39/37 (M). 51/40/38 (R).
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #9508. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #9508

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    Caspase-9 (C9) Mouse mAb (BSA and Azide Free) detects endogenous levels of the pro form of caspase-9 as well as cleaved fragments.

    Species Reactivity:

    Human, Mouse, Rat, Hamster, Monkey

    Source / Purification

    Monoclonal Antibody is produced by immunizing mice with a recombinant human caspase-9 protein.

    Background

    Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing, resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330, producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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