Render Target: STATIC
Render Timestamp: 2024-12-13T11:06:43.905Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-08-01 15:28:39.916
Product last modified at: 2024-10-28T13:30:09.253Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. Cathepsin L Antibody

Cathepsin L Antibody #71298

Filter:
  • WB

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 25-42
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cathepsin L Antibody recognizes endogenous levels of total cathepsin L protein. The antibody detects full-length cathepsin L pro-enzyme in addition to the processed (active) form of cathepsin L.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro229 of human cathepsin L protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Cathepsin L belongs to the C1 family of peptidases, a large and diverse family of cysteine (thiol) proteases that play fundamental roles in protein processing and degradation. The catalytic activity of C1 peptidases maps to a nucleophilic cysteine thiol, usually found within a catalytic dyad or triad (1). Cathepsin L, initially synthesized as procathepsin L before undergoing proteolytic cleavage to its active form, is a ubiquitously expressed thiol protease, expressed primarily in lysosomes but which is also secreted by some cell types. Its diverse functions include general protein catabolism and autophagy (2), antigen processing (3), promoting angiogenesis (4), and extracellular matrix degradation (5,6). An isoform of cathepsin L has also been reported in the nucleus, where it was shown to be involved in the regulation of transcription factor activity (7). Targeted deletion of the Ctsl gene in mice embryos was non-lethal, but resulted in periodic hair loss and skin defects, including epidermal hyperplasia, acanthosis, and hyperkeratosis (8). More recently, research studies have reported that cathepsin L is involved in viral protein processing during viral replication, and that cathepsin L inhibitors may have utility in combating infection by SARS-CoV-2 and related coronaviruses (9).

    Database Links

    UniProt ID: P07711


    Entrez-Gene Id: 1514


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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    KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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