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Render Timestamp: 2024-12-20T12:08:12.910Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-06-28 20:07:09.397
Product last modified at: 2024-12-17T12:15:09.039Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. CCN3 Antibody

CCN3 Antibody #8767

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 43
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CCN3 Antibody recognizes endogenous levels of total CCN3 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg118 of human CCN3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    CCN3, also named NOV (Nephroblastoma overexressed), belongs to the CCN (Cyr61, Ctgf, NOV) family of proteins. It is a cystine-rich secretory protein that associates with components of the extracellular matrix. Like other CCN family members, CCN3 is capable of mediating diverse biological functions through its four distinct domains, which enable binding to numerous protein partners (1-5).

    CCN3 modulates bone turnover through various mechanisms and is implicated in the progression of primary bone cancers such as osteosarcoma and chondrosarcoma (6-8). Research has shown that CCN3 is also involved in the bone metastasis of melanoma, breast cancer, and prostate cancers (9-11). Recently, CCN3 was reported to play an important role in stem cell renewal (12). CCN3 is normally expressed in both embryonic and adult tissues (13,14). The activity of CCN3 is influenced by post translational modifications and proteolytic cleavage (15,16).
    1. Perbal, B. (2001) Mol Pathol 54, 57-79.
    2. Brigstock, D.R. et al. (2003) Mol Pathol 56, 127-8.
    3. Leask, A. and Abraham, D.J. (2006) J Cell Sci 119, 4803-10.
    4. Yeger, H. and Perbal, B. (2007) J Cell Commun Signal 1, 159-64.
    5. McCallum, L. and Irvine, A.E. (2009) Blood Rev 23, 79-85.
    6. Perbal, B. et al. (2008) Clin Cancer Res 14, 701-9.
    7. Tzeng, H.E. et al. (2011) J Cell Physiol 226, 3181-9.
    8. Yang, W. et al. (2011) Hum Reprod 26, 2850-60.
    9. Vallacchi, V. et al. (2008) Cancer Res 68, 715-23.
    10. Ouellet, V. et al. (2011) Am J Pathol 178, 2377-88.
    11. Chen, P.C. et al. (2012) Carcinogenesis 33, 937-45.
    12. Gupta, R. et al. (2007) Science 316, 590-3.
    13. Burren, C.P. et al. (1999) J Clin Endocrinol Metab 84, 1096-103.
    14. Kocialkowski, S. et al. (2001) Anat Embryol (Berl) 203, 417-27.
    15. Perbal, B. et al. (1999) Proc Natl Acad Sci U S A 96, 869-74.
    16. Su, B.Y. et al. (2001) Mol Pathol 54, 184-91.

    Database Links

    UniProt ID: P48745


    Entrez-Gene Id: 4856


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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