Render Target: STATIC
Render Timestamp: 2024-11-21T13:51:24.435Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:59:50.424
Product last modified at: 2024-10-23T14:15:10.673Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD10/Neprilysin (F4P6H) Rabbit mAb #58145

Filter:
  • WB
  • IHC
  • IF
  • F

    Supporting Data

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 100
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunohistochemistry (Paraffin) 1:50
    Immunofluorescence (Frozen) 1:200 - 1:800
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800
    Flow Cytometry (Fixed/Permeabilized) 1:400 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #67330.

    Protocol

    Specificity / Sensitivity

    CD10/Neprilysin (F4P6H) Rabbit mAb recognizes endogenous levels of total CD10/Neprilysin protein.

    Species Reactivity:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu493 of mouse CD10/Neprilysin protein.

    Background

    CD10, also known as Neprilysin, common acute lymphoblastic leukemia antigen (CALLA), or neutral endopeptidase (NEP), is a zinc-dependent transmembrane metallo-endopeptidase. CD10 cleaves peptides at the N-terminal side of hydrophobic amino acid residues and deactivates a variety of signaling peptides (1,2). CD10 was shown to be one of the markers for a natural killer cell-restricted progenitor in fetal and adult tissues (3). Studies also suggest CD10 as a phenotypic marker to distinguish mature neutrophils from immature neutrophils in patients with inflammation (2). In addition, CD10+GPR77+ carcinoma-associated fibroblasts (CAFs) promote tumor formation and chemoresistance (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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