CD109 (E8L2W) Rabbit mAb #48695
- WB
- IP
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 190 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Flow Cytometry (Live) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
CD109 (E8L2W) Rabbit mAb recognizes endogenous levels of total CD109 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly653 of human CD109 protein.
Background
CD109 is a glycosylphosphatidylinositol (GPI)-linked glycoprotein that belongs to the alpha2-macroglobulin family of thioester containing proteins (1). CD109 is associated with TGF-beta receptor I (TbRI) and inhibits TGF-beta signaling (2,3). Cleavage of CD109 at its Furin cleavage site results in the release of its large amino-terminal domain, which then binds to the TGF-beta receptor I to inhibit TGF-beta signaling (4-7). CD109 is expressed on a subset of CD34+ bone marrow cells and mesenchymal stem cells, activated platelets, activated T cells, endothelial cells, and a wide variety of tumors (8-10). Elevated CD109 expression has been considered a diagnostic/prognostic marker for several types of cancers (11-14).
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- Zhang, J.M. et al. (2015) Biochem Biophys Res Commun 459, 252-8.
- Hagiwara, S. et al. (2010) Oncogene 29, 2181-91.
- Litvinov, I.V. et al. (2011) Exp Dermatol 20, 627-32.
- Zhou, S. et al. (2017) Oncotarget 8, 95632-47.
- Li, C. et al. (2016) Biochem J 473, 537-47.
- Hashimoto, M. et al. (2004) Oncogene 23, 3716-20.
- Sato, T. et al. (2007) Pathol Int 57, 719-24.
- Qi, R. et al. (2018) J Transl Med 16, 88.
- Emori, M. et al. (2015) J Surg Oncol 111, 975-9.
- Jia, W. et al. (2016) Oncotarget 7, 55328-42.
- Yokoyama, M. et al. (2017) Int J Hematol 105, 614-22.
- Chuang, C.H. et al. (2017) Nat Med 23, 291-300.
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