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Render Timestamp: 2025-01-21T04:19:42.784Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:56:06.758
Product last modified at: 2025-01-01T09:02:56.362Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD133 (A8N6N) Mouse mAb #60577

Filter:
  • F
Flow Cytometry Image 1: CD133 (A8N6N) Mouse mAb
Flow cytometric analysis of live 293T cells (blue) and Weri-Rb1 cells (green) using CD133 (A8N6N) Mouse mAb (solid lines) or a concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed lines). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

To Purchase # 60577

Cat. #

Size

60577T
20 µl
60577S
100 µl

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Mouse IgG3
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Flow Cytometry (Live) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CD133 (A8N6N) Mouse mAb recognizes endogenous levels of total CD133 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with cells overexpressing human CD133 protein.

Background

CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells (1). Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis (2). Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells (3-7). A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans (8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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