Render Target: STATIC
Render Timestamp: 2024-12-13T11:59:33.772Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-16 20:01:09.313
Product last modified at: 2024-10-07T19:45:09.485Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD15/SSEA1 (MC480) Mouse mAb (BSA and Azide Free) #74180

Filter:
  • IF
  • F

    Supporting Data

    REACTIVITY
    SENSITIVITY Endogenous
    MW (kDa) N/A
    Source/Isotype Mouse IgM
    Application Key:
    • IF-Immunofluorescence 
    • F-Flow Cytometry 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #4744. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #4744.

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    CD15/SSEA1 Mouse mAb (BSA and Azide Free) detects endogenous levels of SSEA1 antigen.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with F9 teratoma cells (X-irradiated).

    Background

    SSEA1 antibody detects a lactoseries oligosaccharide antigen that is expressed on the surface of mouse embryonal carcinoma and embryonic stem cells (1). This antigen is also found on early mouse embryos and both mouse and human germ cells, but is absent on human embryonic stem and carcinoma cells. Expression of SSEA1 in these human cell types increases upon differentiation, while on the mouse cell types differentiation leads to decreased expression (2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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