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Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-08-01 15:28:34.682
Product last modified at: 2025-01-01T09:06:50.482Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

CD177 Antibody #25068

Filter:
  • WB
Western Blotting Image 1: CD177 Antibody
Western blot analysis of extracts from C2C12 cells and mouse bone marrow tissue using CD177 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 25068

Cat. # Size Qty. Price
25068S 100 µl
$306

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 105
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CD177 Antibody recognizes endogenous levels of total CD177 protein.

Species Reactivity:

Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val703 of mouse CD177 protein.

Background

Cluster of differentiation 177 antigen (CD177, also known as human neutrophil alloantigen 2a and NB1), is a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated transmembrane protein expressed primarily on the surface of neutrophils (1,2). Expression of CD177 can be induced by granulocyte-colony-stimulating factor and during pregnancy or bacterial infection (3-5). CD177 mediates adhesion and transendothelial migration of neutrophils via interaction with CD31/PECAM-1 and integrin β2 on endothelial cells (6,7). CD177 also promotes the cell surface expression of proteinase 3 (PR3), which may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (8-10). In association with CD11b/ITGAM, integrin β2, and PR3, CD177 promotes activation of TNF-α primed neutrophils including degranulation and superoxide production (11). CD177 is a polymorphic gene that has been linked to several important clinical diseases, including polycythemia vera, Wegener’s granulomatosis, and immune mediated neonatal neutropenia (12-14).
For Research Use Only. Not For Use In Diagnostic Procedures.
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