CD200 (E2K4C) Rabbit mAb #69858
- WB
- IP
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45-50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Flow Cytometry (Live) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
CD200 (E2K4C) Rabbit mAb recognizes endogenous levels of total CD200 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with human CD200 recombinant protein and reacts with an epitope surrounding Pro189.
Background
CD200 (OX2) and CD200R (OX2R) are membrane glycoprotein members of the Ig superfamily (1-3). CD200 is expressed by a range of cells, including neurons, epithelial cells, endothelial cells, fibroblasts, and lymphoid cells, while its receptor, CD200R, is found on myeloid and T cells (1-5). Interaction between CD200 and CD200R downregulates macrophage function and plays a role in immunosuppression and regulation of anti-tumor immune responses (3-7).
- Barclay, A.N. (1981) Immunology 44, 727-36.
- McCaughan, G.W. et al. (1987) Immunogenetics 25, 329-35.
- Wright, G.J. et al. (2000) Immunity 13, 233-42.
- Hoek, R.M. et al. (2000) Science 290, 1768-71.
- Wright, G.J. et al. (2003) J Immunol 171, 3034-46.
- Kretz-Rommel, A. et al. (2007) J Immunol 178, 5595-605.
- Fallarino, F. et al. (2004) J Immunol 173, 3748-54.
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