CD23 (E1R4G) Rabbit mAb #87960
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:800 - 1:3200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #89186.
Protocol
Specificity / Sensitivity
CD23 (E1R4G) Rabbit mAb recognizes endogenous levels of total CD23 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala118 of human CD23 protein.
Background
CD23, also known as FcεRII, is a trimeric type II transmembrane glycoprotein member of the calcium-dependent (C-type) lectin family (1). CD23 is expressed in B and T lymphocytes, monocytes, polymorphonuclear leukocytes, follicular dendritic cells, intestinal epithelial cells, and bone marrow stromal cells (2-8). CD23 was first identified as a low-affinity receptor for IgE and the engagement of membrane bound CD23 leads to suppressed IgE production by B lymphocytes (9). CD23 can be cleaved to form a soluble protein which can drive cytokine release in monocytic cells, nitric oxide (NO) production, and the synthesis of cyclic adenosine 3′5′-monophosphate (cAMP) (10). Soluble CD23 signals through integrins, activating MAPK and NF-κB pathways (11). CD23 is a useful marker in the prognosis of neoplastic disease, is elevated in a variety of autoimmune and inflammatory conditions, and is being investigated as a therapeutic target for IgE-mediated allergy, arthritis, and B cell chronic lymphocytic leukemia (CLL) (12).
- Wurzburg, B.A. et al. (2006) Structure 14, 1049-58.
- Armitage, R.J. et al. (1989) Eur J Immunol 19, 31-5.
- Vercelli, D. et al. (1988) J Exp Med 167, 1406-16.
- Yamaoka, K.A. et al. (1996) Int Immunol 8, 479-90.
- Capron, M. et al. (1992) Immunol Res 11, 252-9.
- Rieber, E.P. et al. (1993) Adv Exp Med Biol 329, 393-8.
- Yu, L.C. et al. (2003) Am J Physiol Gastrointest Liver Physiol 285, G223-34.
- Fourcade, C. et al. (1992) Eur Cytokine Netw 3, 539-43.
- Suter, U. et al. (1987) Nucleic Acids Res 15, 7295-308.
- Rezzonico, R. et al. (2001) Blood 97, 2932-40.
- Rezzonico, R. et al. (2000) Blood 95, 3868-77.
- Acharya, M. et al. (2010) Clin Exp Immunol 162, 12-23.
Limited Uses
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