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Product last modified at: 2025-01-01T09:06:19.933Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

CD248 Antibody #20858

Filter:
  • WB
Western Blotting Image 1: CD248 Antibody
Western blot analysis of extracts from SJSA-1 and COLO 205 cells using CD248 Antibody (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, COLO 205 cells are low or negative for CD248 expression. Some lower bands can be apparent on the western blot, appearing beneath the CD248 band. We believe these are incompletely post-translationally modified forms of CD248.

To Purchase # 20858

Cat. # Size Qty. Price Ships
20858S 100 µl
$306

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 190
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CD248 Antibody recognizes endogenous levels of total CD248 protein.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro403 of human CD248 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CD248, also known as Endosialin and TEM1, is a stromal cell marker expressed on activated mesenchymal cells including fibroblasts and pericytes. Not normally detectable in most adult tissues, it is highly expressed in lymphoid tissues during development, and in disease states where increased stromal cell proliferation and migration are evident (1-3). CD248 is known to be upregulated in breast cancer, brain tumors (4-6), and other malignancies including sarcoma (12), and it has been implicated in sprouting angiogenesis and vasculogenesis (7). It is thought that the CD248 gene is upregulated in response to hypoxic conditions in the tumor environment through HIF2 activation (8). Interestingly, CD248 is found to be highly expressed in activated fibroblasts. In liver fibrosis for example, CD248 marks the Hepatic Stellate Cells, the activated cells responsible for matrix production (9,10). In kidney fibrosis, CD248 marks the key effector cells within the fibrotic stroma including pericytes, myofibroblasts, and stromal fibroblasts (11), and in Idiopathic Pulmonary Fibrosis, CD248 may be a marker for severity of disease (12). CD248 has become a target of interest for pharmaceutical intervention in a wide scope of diseases (13).
For Research Use Only. Not For Use In Diagnostic Procedures.
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