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Product last modified at: 2025-01-01T09:02:39.092Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD31 (PECAM-1) (F8M3S) Rabbit mAb (BSA and Azide Free) #71397

Filter:
  • WB
  • IHC
Western Blotting Image 1: CD31 (PECAM-1) (F8M3S) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from SUP-B15 and SiHa cells using CD31 (PECAM-1) (F8M3S) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of CD31 (PECAM-1) protein in SiHa cells is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.

To Purchase # 71397

Cat. # Size Qty. Price Ships
71397SF 100 µg
$843

Supporting Data

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa) 135
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • Mk-Monkey 

Product Information

Product Usage Information

This product is the carrier free version of product #95462. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #95462

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

CD31 (PECAM-1) (F8M3S) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD31 (PECAM-1) protein. Non-specific staining was observed of human testis by immunohistochemistry.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD31 (PECAM-1) protein.

Background

CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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