CD4 (MSVA-004R) Rabbit mAb #99785

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	55
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:50 - 1:200

Storage

Supplied in a PBS-based buffer with ~1% BSA and less than 0.1% sodium azide. Stable for 24 months when stored at 4°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CD4 (MSVA-004R) Rabbit mAb recognizes endogenous levels of CD4 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human CD4 protein.

Background

Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part, and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and recruits the tyrosine kinase Lck, which is essential for T cell activation (1).

Zamoyska, R. (1994) Immunity 1, 243-6.

Database Links

UniProt ID: P01730

Entrez-Gene Id: 920

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For Research Use Only. Not For Use In Diagnostic Procedures.

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