Render Target: STATIC
Render Timestamp: 2024-11-21T14:05:34.230Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:59:14.911
Product last modified at: 2024-11-12T18:45:14.944Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD62L/L-Selectin (E7X9I) Rabbit mAb #58225

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 60-100
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #98131.

    Protocol

    Specificity / Sensitivity

    CD62L/L-Selectin (E7X9I) Rabbit mAb recognizes endogenous levels of total CD62L/L-Selectin protein. Non-specific staining was observed in the surface epithelium of tonsil by immunohistochemistry.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with human CD62L/L-Selectin recombinant protein.

    Background

    L-Selectin (CD62L, MEL-14, LAM1, SELL) is a cell adhesion molecule responsible for homing and mediating the binding of lymphocytes to high endothelial venules (HEV) in secondary lymphoid tissues (1-5). It is a commonly used marker for distinguishing naive and memory T cells from effector T cells (6).

    Due to cell type-specific glycosylation, the actual molecular weight of L-Selectin differs between cell types and can range from 60 kDa in lymphocytes up to 100 kDa in neutrophils (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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