Render Target: STATIC
Render Timestamp: 2024-11-21T13:11:28.901Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-01 15:31:12.745
Product last modified at: 2024-11-15T20:45:07.542Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD68 (E3O7V) Rabbit mAb #97778

Filter:
  • WB
  • IHC
  • IF
  • F

    Supporting Data

    REACTIVITY M R Hm
    SENSITIVITY Endogenous
    MW (kDa) 70-80, 130-140, 200
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    IHC Leica Bond 1:600 - 1:2400
    Immunohistochemistry (Paraffin) 1:150 - 1:600
    Immunofluorescence (Frozen) 1:400 - 1:1600
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:400
    Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:200
    Flow Cytometry (Live) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #29176.

    Protocol

    Specificity / Sensitivity

    CD68 (E3O7V) Rabbit mAb recognizes endogenous levels of total CD68 protein. Staining of uncertain specificity in mouse endometrial epithelium has been observed by immunohistochemistry.

    Species Reactivity:

    Mouse, Rat, Hamster

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with mouse CD68 recombinant protein and reacts with an epitope surrounding Gly113.

    Background

    CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (1,2). It is found on the plasma membrane, as well as endosomal and lysosomal membranes (1-3). It is proposed to bind OxLDL and has been observed as a homodimer (3,4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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