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Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:15:11.560
Product last modified at: 2025-03-20T19:30:09.598Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD68 (E3O7V) Rabbit mAb #97778

Filter:
  • WB
  • IHC
  • IF
  • F
Western Blotting Image 1: CD68 (E3O7V) Rabbit mAb
Western blot analysis of extracts from various mouse cells using CD68 (E3O7V) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

To Purchase # 97778

Cat. # Size Qty. Price
97778T 20 µl
$153
97778S 100 µl
$357

Supporting Data

REACTIVITY M R Hm
SENSITIVITY Endogenous
MW (kDa) 70-80, 130-140, 200
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • M-Mouse 
  • R-Rat 
  • Hm-Hamster 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
IHC Leica Bond 1:600 - 1:2400
Immunohistochemistry (Paraffin) 1:150 - 1:600
Immunofluorescence (Frozen) 1:400 - 1:1600
Immunofluorescence (Immunocytochemistry) 1:200 - 1:400
Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:200
Flow Cytometry (Live) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #29176.

Protocol

Specificity / Sensitivity

CD68 (E3O7V) Rabbit mAb recognizes endogenous levels of total CD68 protein. Staining of uncertain specificity in mouse endometrial epithelium has been observed by immunohistochemistry.

Species Reactivity:

Mouse, Rat, Hamster

Source / Purification

Monoclonal antibody is produced by immunizing animals with mouse CD68 recombinant protein and reacts with an epitope surrounding Gly113.

Background

CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (1,2). It is found on the plasma membrane, as well as endosomal and lysosomal membranes (1-3). It is proposed to bind OxLDL and has been observed as a homodimer (3,4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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