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Product last modified at: 2025-01-01T09:04:04.328Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

CD84 Antibody #41033

Filter:
  • WB
  • IP
Western Blotting Image 1: CD84 Antibody
Western blot analysis of extracts from J774A.1 cells, mouse bone marrow-derived dendritic cells (Mouse BMDCs), and C2C12 cells using CD84 Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Mouse bone marrow was extracted and then treated with Mouse Granulocyte Macrophage Colony Stimulating Factor (mGM-CSF) #5191 (20 ng/ml, 7 d).

To Purchase # 41033

Cat. # Size Qty. Price
41033S 100 µl
$306

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 45-80
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CD84 Antibody recognizes endogenous levels of total CD84 protein. This antibody cross-reacts with an unidentified protein of 17 kDa.

Species Reactivity:

Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala322 of mouse CD84 protein. Antibodies are purified by peptide affinity chromatography.

Background

Cluster of Differentiation 84 (CD84), also known as signaling lymphocytic activation molecule family member 5 (SLAMF5), is a membrane glycoprotein expressed by numerous immune cell types, including hematopoietic progenitor cells, T cells, B cells, NK cells, macrophages, monocytes, dendritic cells, platelets, mast cells, and thymocytes (1-9). CD84 is a homophilic adhesion molecule involved in modulating the activation, differentiation, proliferation, and cytotoxicity of a wide variety of immune cells, and thus is involved in the regulation and interconnection of both the innate and adaptive immune response (6-14). Certain CD84 activities are regulated by the presence or absence of small cytoplasmic adaptor proteins, SH2D1A/SAP and/or SH2D1B/EAT-2 (5,14). Alternate splicing results in multiple transcript variants.
For Research Use Only. Not For Use In Diagnostic Procedures.
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