CD8α (D8A8Y) Rabbit mAb #85336

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Supporting Data

REACTIVITY	H Mk
SENSITIVITY	Endogenous
MW (kDa)	29
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IHC-Immunohistochemistry
F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Immunohistochemistry (Paraffin)	1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized)	1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #81575.

Protocol

Specificity / Sensitivity

CD8α (D8A8Y) Rabbit mAb recognizes endogenous levels of total CD8α protein. Monkey species reactivity is Immunohistochemistry (Paraffin) only.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CD8α protein.

Background

Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1).

Zamoyska, R. (1994) Immunity 1, 243-46.

Database Links

UniProt ID: P01732

Entrez-Gene Id: 925

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