CD8β (D7C8H) Rabbit mAb #94127

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Supporting Data

REACTIVITY	M
SENSITIVITY	Endogenous
MW (kDa)	27-32
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CD8β (D7C8H) Rabbit mAb recognizes endogenous levels of total CD8β protein. This antibody detects an 11 kDa band of unknown origin in some cell lines.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with mouse CD8β recombinant protein.

Background

Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1).

Zamoyska, R. (1994) Immunity 1, 243-46.

Database Links

UniProt ID: P10300

Entrez-Gene Id: 12526

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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