Render Target: STATIC
Render Timestamp: 2024-12-20T11:34:03.853Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:57:51.435
Product last modified at: 2024-12-17T18:49:51.033Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD96 (E4B8Y) Rabbit mAb #18052

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 100-200
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CD96 (E4B8Y) Rabbit mAb recognizes endogenous levels of total mouse CD96 protein. CD96 (E4B8Y) Rabbit mAb is more sensitive by immunoprecipitation for the unglycosylated form of CD96 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu368 of mouse CD96 protein.

    Background

    Cluster of Differentiation 96 (CD96), also known as T cell-activated increased late expression protein (TACTILE), is a heavily glycosylated type I transmembrane protein belonging to the immunoglobulin superfamily (1-3). CD96 is expressed on peripheral T cells and natural killer (NK) cells, and is strongly upregulated on these cell types after activation (1,4). It is also expressed on transformed T cells, and at very low levels on activated B cells (1,5,6). CD96 interacts with the receptor ligand PVR (CD155), and is involved in adhesion of activated T and NK cells to target cells during the late phase of the immune response (2,4). CD96 competes with DNAM-1 (CD226) for binding to PVR (CD155) in order to negatively regulate T and NK cell activity, and thus blockade of CD96 in order to induce anti-tumor immune responses is investigated for potential immunotherapeutic intervention in cancer (7-9). Alternative splicing occurs at this locus, and two transcript variants encoding distinct isoforms with variable functions have been identified (1,10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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