CD96 (F2I1M) Rabbit mAb #38515
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100, 160 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
CD96 (F2I1M) Rabbit mAb recognizes endogenous levels of total CD96 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracullular domain of human CD96 protein.
Background
Cluster of Differentiation 96 (CD96), also known as T cell-activated increased late expression protein (TACTILE), is a heavily glycosylated type I transmembrane protein belonging to the immunoglobulin superfamily (1-3). CD96 is expressed on peripheral T cells and natural killer (NK) cells, and is strongly upregulated on these cell types after activation (1,4). It is also expressed on transformed T cells, and at very low levels on activated B cells (1,5,6). CD96 interacts with the receptor ligand PVR (CD155), and is involved in adhesion of activated T and NK cells to target cells during the late phase of the immune response (2,4). CD96 competes with DNAM-1 (CD226) for binding to PVR (CD155) in order to negatively regulate T and NK cell activity, and thus blockade of CD96 in order to induce anti-tumor immune responses is investigated for potential immunotherapeutic intervention in cancer (7-9). Alternative splicing occurs at this locus, and two transcript variants encoding distinct isoforms with variable functions have been identified (1,10).
- Wang, P.L. et al. (1992) J Immunol 148, 2600-8.
- Seth, S. et al. (2007) Biochem Biophys Res Commun 364, 959-65.
- Georgiev, H. et al. (2018) Front Immunol 9, 1072.
- Fuchs, A. et al. (2004) J Immunol 172, 3994-8.
- Burger, R. et al. (1999) Leuk Res 23, 19-27.
- Gramatzki, M. et al. (1998) Exp Hematol 26, 1209-14.
- Chan, C.J. et al. (2014) Nat Immunol 15, 431-8.
- Blake, S.J. et al. (2016) Cancer Discov 6, 446-59.
- Brooks, J. et al. (2018) Cancer Res 78, 475-88.
- Meyer, D. et al. (2009) J Biol Chem 284, 2235-44.
Limited Uses
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