Render Target: STATIC
Render Timestamp: 2024-11-29T11:22:06.449Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-04-05 20:25:53.375
Product last modified at: 2024-10-02T11:00:11.078Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

CD99 Antibody #16121

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 30
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CD99 Antibody recognizes endogenous levels of total CD99 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln71 of human CD99 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    CD99 is a transmembrane protein involved in many cellular functions, including cell adhesion and migration, endocytosis and exocytosis, and intracellular protein trafficking. It is highly expressed in all leukocyte lineages, Sertoli cells, granulosa cells, and pancreatic islet cells (1,2). Due to alternative splicing, there are two isoforms of CD99 that differ at the carboxy terminus. CD99 Type I (CD99wt) is the full-length form containing 185 amino acids and CD99 Type II (CD99sh) contains 161 amino acids. Their expression is differentially regulated and they may have opposite functions in different contexts (3,4). CD99 is expressed in many types of tumors and has been used for differential diagnosis of conventional Ewing sarcoma. It has been actively pursued as a therapeutic target (5,6). On the other hand, CD99 may also play a tumor suppressor role in other tumors, such as Hodgkin’s lymphomas, osteosarcomas, and pancreatic tumors (6,7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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