CD99 (PCB1) Mouse mAb #29983
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| IHC Leica Bond | 1:50 - 1:200 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
CD99 (PCB1) Mouse mAb recognizes endogenous levels of CD99 protein. Non-specific staining was observed in brain by immunohistochemistry.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant protein corresponding to 101 amino acids of the N–terminal region of human CD99 protein.
Background
CD99 is a transmembrane protein involved in many cellular functions, including cell adhesion and migration, endocytosis and exocytosis, and intracellular protein trafficking. It is highly expressed in all leukocyte lineages, Sertoli cells, granulosa cells, and pancreatic islet cells (1,2). Due to alternative splicing, there are two isoforms of CD99 that differ at the carboxy terminus. CD99 Type I (CD99wt) is the full-length form containing 185 amino acids and CD99 Type II (CD99sh) contains 161 amino acids. Their expression is differentially regulated and they may have opposite functions in different contexts (3,4). CD99 is expressed in many types of tumors and has been used for differential diagnosis of conventional Ewing sarcoma. It has been actively pursued as a therapeutic target (5,6). On the other hand, CD99 may also play a tumor suppressor role in other tumors, such as Hodgkin’s lymphomas, osteosarcomas, and pancreatic tumors (6,7).
- Muller, W.A. (2016) Immunol Rev 273, 61-75.
- Pasello, M. et al. (2018) J Cell Commun Signal 12, 55-68.
- Alberti, I. et al. (2002) FASEB J 16, 1946-8.
- Scotlandi, K. et al. (2007) Oncogene 26, 6604-18.
- Scotlandi, K. (2006) Adv Exp Med Biol 587, 13-22.
- Manara, M.C. et al. (2018) Genes (Basel) 9, 159.
- Manara, M.C. et al. (2006) Mol Biol Cell 17, 1910-21.
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