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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

cdc2 Antibody #77055

Filter:
  • WB
Western Blotting Image 1: cdc2 Antibody
Western blot analysis of extracts from HeLa, 3T3, and C6 cells using cdc2 Antibody.

To Purchase # 77055

Cat. # Size Qty. Price
77055S 100 µl
$350

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 34
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

cdc2 Antibody detects endogenous levels of total cdc2 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp289 of human cdc2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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