Cdc7 Antibody #3603
- WB
Supporting Data
| REACTIVITY | H M R Hm |
| SENSITIVITY | Endogenous |
| MW (kDa) | 64 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Hm-Hamster
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
The import of cdc7 to the nucleus is regulated by importin-β (5) and its binding to the origin of replication is dependent on the regulation of its localization via three domains, a nuclear localization sequence (NLS), a nuclear retention sequence (NRS) and a nuclear export sequence (NES) (6).
Expression of cdc7 and dbf4 has been shown to be increased in human cancer cell lines and tissue (7); a chemical inhibitor of cdc7 blocks initiation of DNA replication and causes apoptosis in cancer cells (8).
Cdc7 is also involved in activating ATR/Chk1 in response to DNA damage (9,10).
- Okuno, Y. et al. (2001) EMBO J 20, 4263-77.
- McNairn, A.J. et al. (2005) Exp Cell Res 308, 345-56.
- Bell, S.P. and Dutta, A. (2002) Annu Rev Biochem 71, 333-74.
- Tsuji, T. et al. (2006) Mol Biol Cell 17, 4459-72.
- Kim, B.J. and Lee, H. (2006) J Biol Chem 281, 12041-9.
- Kim, B.J. et al. (2007) J Biol Chem 282, 30029-38.
- Bonte, D. et al. (2008) Neoplasia 10, 920-31.
- Montagnoli, A. et al. (2008) Nat Chem Biol 4, 357-65.
- Heffernan, T.P. et al. (2007) J Biol Chem 282, 9458-68.
- Kim, J.M. et al. (2008) Oncogene 27, 3475-82.
Limited Uses
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