Render Target: STATIC
Render Timestamp: 2024-11-21T13:53:34.961Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-05-10 22:31:14.823
Product last modified at: 2024-11-19T01:15:07.938Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CDK7 (MO1) Mouse mAb #2916

Filter:
  • WB
  • IP
  • IHC

    Supporting Data

    REACTIVITY H R
    SENSITIVITY Endogenous
    MW (kDa) 40
    Source/Isotype Mouse IgG2b
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:2000
    Immunoprecipitation 1:200
    Immunohistochemistry (Paraffin) 1:500

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CDK7 (MO1) Mouse mAb detects endogenous levels of total CDK7 protein. The antibody does not cross-react with other CDKs.

    Species Reactivity:

    Human, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant human CDK7. The epitope corresponds to a region surrounding Gln320 of human CDK7.

    Background

    CDK-activating kinase (CAK) is a complex of CDK7 and cyclin H. The complex is involved in cell cycle regulation by phosphorylating an activating residue in the T-loop domain of cdks (1). Regulation of CAK activity is mediated by T-loop phosphorylation and by association with MAT1, both of which enhance its kinase activity toward the CTD of RNA polymerase II (2,3) and other substrates such as p53 (4). CAK is an essential component of the transcription complex TFIIH and may interact directly with TFIIH helicases (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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