CELA2A (E1D6G) Rabbit mAb #23050

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	26
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CELA2A (E1D6G) Rabbit mAb recognizes endogenous levels of total CELA2A protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly176 of human CELA2A protein.

Background

Chymotrypsin-like elastase family member 2A (CELA2A) was initially described as an exocrine elastase, produced by exocrine cells of the mammalian pancreas (1,2). Deeper examination revealed additional, lower levels of CELA2A expression by a small subset of pancreatic islet cells and many other tissues, including the adrenal glands, intestine and colon, liver, and white adipose tissue (3). Notably, gene expression datasets have reported aberrant CELA2A expression by other tissues in association with specific disease states (e.g., insulin resistance or Type II diabetes) (3), while increased expression of CELA2A by colonic epithelial cells was reported in patients with inflammatory bowel diseases (4). CELA2A is a secreted protease; in normal physiology, it is released into circulation following an elevation in plasma glucose levels, where it promotes both insulin secretion and degradation, leading to increased insulin sensitivity (3). Importantly, mutations in the CELA2A gene were shown to inhibit the ability of CELA2A to regulate insulin dynamics (3). Collectively, these studies suggest that CELA2A may be an attractive therapeutic target in both metabolic and inflammatory diseases.

Database Links

UniProt ID: P08217

Entrez-Gene Id: 63036

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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