CEP55 (D1L4H) Rabbit mAb #81693
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Protocol
Specificity / Sensitivity
CEP55 (D1L4H) Rabbit mAb recognizes endogenous levels of total CEP55 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val459 of human CEP55 protein.
Background
The 55 kDa centrosomal protein (CEP55) is a widely expressed centrosome and midbody-associated protein that regulates cytokinesis, including completion of the final step during cytokinesis known as abscission (1,2). CEP55 activity during abscission is negatively regulated by p53 through Polo-like kinase 1 (3,4). The breast and ovarian cancer DNA repair protein BRCA2 interacts with CEP55 and plays a regulatory role during abscission (5). Research studies demonstrate that CEP55 is also involved in the regulation of Akt signaling, autophagy, and may be a biomarker in human cancer (reviewed in 6). The correlated overexpression of CEP55, the transcription factor FoxM1, and the HELLS helicase is seen in head and neck squamous cell carcinoma (7,8). Additional studies demonstrate that CEP55 expression regulates cell proliferation in gastric carcinoma (9).
Limited Uses
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