ChAT (E4F9G) Rabbit mAb #27269

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	71
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ChAT (E4F9G) Rabbit mAb recognizes endogenous levels of total human, mouse, and rat ChAT protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human ChAT protein.

Background

Choline O-acetyltransferase (ChAT) catalyzes the synthesis of the neurotransmitter acetylcholine (ACh) from acetyl CoA and choline in the central and peripheral nervous system (1). In mammals, ACh is the primary neurotransmitter used at the neuromuscular junction, released by motor neurons to activate muscles. Neurons that release ACh are known as cholinergic neurons, which express high levels of ChAT. In addition to motor neurons, cholinergic neurons are present in the brain, particularly in the basal forebrain as well as other regions of the brain, including striatal interneurons. Reduction in ChAT, indicative of degeneration of cholinergic neurons, is correlated with cognitive decline in several neurodegenerative diseases, including Alzheimer’s disease (2).

Database Links

UniProt ID: P28329

Entrez-Gene Id: 1103

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For Research Use Only. Not For Use In Diagnostic Procedures.

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