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Inquiry Info. # 4297

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Supporting Data

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)	80
SOURCE	Rabbit

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CHFR Antibody detects endogenous levels of total CHFR protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence around Lys646 of human CHFR protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Checkpoint with forkhead and RING finger domains protein (CHFR) is an E3 ubiquitin-protein ligase that regulates cell cycle progression. In response to microtubule stress, CHFR delays the transition into mitosis by excluding cyclin B1 from the nucleus prior to chromosome condensation (1). Marked reduction of CHFR expression was detected in primary tumors and decreased CHFR expression was linked to promoter hypermethylation (1-4). Restoration of CHFR expression by treatment with the microtubule stress agent nocodazole and the methyltransferase inhibitor 5-aza-2'-deoxycytidine has been reported (4,5).

Database Links

UniProt ID: Q96EP1

Entrez-Gene Id: 55743

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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CHFR Antibody #4297