Render Target: STATIC
Render Timestamp: 2024-12-26T11:16:53.648Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:28:27.272
Product last modified at: 2024-10-14T11:15:08.533Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

CHMP2A Antibody #84706

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 28, 31
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CHMP2A Antibody recognizes endogenous levels of total CHMP2A protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CHMP2A protein. Antibodies are purified by peptide affinity chromatography.

    Background

    CHMP2A is a component of ESCRT-III (endosomal sorting complex required for transport-III) (1,2). The ESCRT system is comprised of multiple protein complexes denoted as ESCRT-0, -I, -II, and -III, and VPS4 ATPase that are recruited to various cellular membranes and function fission reactions (3,4). Yeast contains four core ESCRT-III subunits: Vps20, Snf7, Vps24, and Vps2. Humans share these subunits and additionally have four Snf7 paralogs (CHMP4A-D) and two Vps2 paralogs (CHMP2A and B). The ESCRT-III complex comprising CHMP2A is involved in various membrane events, including multivesicular bodies, viral budding, cytokinesis, nuclear membrane reformation, and phagophore closure during autophagy and mitophagy (5-9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.