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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CHMP4B (E2L7M) Rabbit mAb #35644

Filter:
  • WB
  • IP
Western blot analysis of extracts from various cell lines using CHMP4B (E2L7M) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of CHMP4B protein in KU812 cells is consistent with the predicted expression pattern.

To Purchase # 35644

Cat. # Size Qty. Price
35644T 20 µl
$153
35644S 100 µl
$339

Product Specifications

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 37
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CHMP4B (E2L7M) Rabbit mAb recognizes endogenous levels of total CHMP4B protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn219 of human CHMP4B protein.

Background

CHMP4B, the human homologue of yeast Snf7, interacts with Alix and is a core component of the ESCRT-III complex (endosomal sorting for transport complex III) (1,2). The ESCRT system is comprised of multiple protein complexes denoted as ESCRT-0, -I, -II, -III, and VPS4 ATPase that together contribute to a number of membrane fission events, including multivesicular body (MVB) and exosome formulation, viral budding, abscission during cytokinesis, and plasma membrane repair (3,4). Interestingly, it was found that the ESCRT-III complex, including CHMP4B is recruited to the plasma membrane during the regulated process of necrosis, necroptosis, and thereby controls membrane integrity sustaining these cells and allowing continued signaling and cytokine/chemokine secretion (5). Elevated expression of CHMP4B has been associated with neuronal apoptosis following intracerebral hemorrhage (6). In addition, high CHMP4B expression was associated with increased proliferation and resistance to doxorubicin in hepatocellular carcinoma (7).
For Research Use Only. Not for Use in Diagnostic Procedures.
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