CHMP4B (E2L7M) Rabbit mAb #35644
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 37 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
CHMP4B (E2L7M) Rabbit mAb recognizes endogenous levels of total CHMP4B protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn219 of human CHMP4B protein.
Background
CHMP4B, the human homologue of yeast Snf7, interacts with Alix and is a core component of the ESCRT-III complex (endosomal sorting for transport complex III) (1,2). The ESCRT system is comprised of multiple protein complexes denoted as ESCRT-0, -I, -II, -III, and VPS4 ATPase that together contribute to a number of membrane fission events, including multivesicular body (MVB) and exosome formulation, viral budding, abscission during cytokinesis, and plasma membrane repair (3,4). Interestingly, it was found that the ESCRT-III complex, including CHMP4B is recruited to the plasma membrane during the regulated process of necrosis, necroptosis, and thereby controls membrane integrity sustaining these cells and allowing continued signaling and cytokine/chemokine secretion (5). Elevated expression of CHMP4B has been associated with neuronal apoptosis following intracerebral hemorrhage (6). In addition, high CHMP4B expression was associated with increased proliferation and resistance to doxorubicin in hepatocellular carcinoma (7).
- Katoh, K. et al. (2003) J Biol Chem 278, 39104-13.
- McCullough, J. et al. (2008) Proc Natl Acad Sci U S A 105, 7687-91.
- Henne, W.M. et al. (2013) Cold Spring Harb Perspect Biol 5(9): a016766.
- McCullough, J. et al. (2013) Annu Rev Biochem 82, 663-92.
- Gong, Y.N. et al. (2017) Cell 169, 286-300.e16.
- Shen, J. et al. (2015) Brain Res 1597, 1-13.
- Hu, B. et al. (2015) Tumour Biol 36, 2569-81.
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