Cell Signaling Technology Logo

Basket Updated

0

Items added

View Featured Offers >>
1% for the planet logo
  1. Products /
  2. Primary Antibodies /
  3. CIRBP Antibody

CIRBP Antibody #13713

We recommend the following alternatives

#

Product Name

68522 CIRBP (D1M8D) Rabbit mAb
Filter:
  • WB
  • IP
Western blot analysis of extracts from human thyroid, human liver, and SH-SY-5Y cells using CIRBP Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
1/2
This product is discontinued

Inquiry Info. # 13713

Please see our recommended alternatives.

Product Specifications

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 18
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CIRBP Antibody recognizes endogenous levels of total CIRBP protein.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly150 of human CIRBP protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cold-induced RNA-binding protein (CIRBP) is a 172-residue, multifunctional sensor protein that was first isolated as a protein induced in mouse fibroblasts cultured at 32ºC (1). Conversely, CIRBP expression decreases in cells or tissues subjected to increased temperature (2). The CIRBP protein is composed of an amino-terminal RNA-binding domain and a carboxyl-terminal, glycine-rich domain (1). Stressful stimuli, such as hypoxia, heat shock, osmotic shock, or oxidative conditions, lead to translocation of CIRBP from the nucleus to cytoplasmic stress granules through a mechanism involving CIRBP methylation-dependent nuclear export (3). CIRBP plays a role in regulating apoptosis and preserving the stemness of neural stem cells at moderately low temperatures (4). Research studies demonstrate that CIRBP contributes to the regulation of circadian rhythm through post-translational modulation of CLOCK expression (5).

Alternate Names

RNA SD

Database Links

UniProt ID: Q14011


Entrez-Gene Id: 1153


Product #13713
Citations: 22

Add/Remove Filters
Sort by:
Publication Date Newest
Applications
Reactivity
Country
Showing 3 Images from 22 Citations
3 of 3
Showing 22 Citations
Applications:
Unspecified
Reactivity:
Unspecified
Applications:
Western Blotting (WB)
Reactivity:
Unspecified
1
Journal:
Proc Natl Acad Sci U S A
Author:
Eric M Desjardins, et al.
Year:
2022
Applications:
Western Blotting (WB)
Reactivity:
Mus musculus (Mouse)
Journal:
Genomics Proteomics Bioinformatics
Author:
Yuejing Jiang, et al.
Year:
2022
Applications:
Western Blotting (WB)
Reactivity:
Unspecified
Applications:
Unspecified
Reactivity:
Unspecified

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.