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Product Name

68522 CIRBP (D1M8D) Rabbit mAb

Filter:

WB
IP

Inquiry Info. # 13713

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	18
SOURCE	Rabbit

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CIRBP Antibody recognizes endogenous levels of total CIRBP protein.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly150 of human CIRBP protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cold-induced RNA-binding protein (CIRBP) is a 172-residue, multifunctional sensor protein that was first isolated as a protein induced in mouse fibroblasts cultured at 32ºC (1). Conversely, CIRBP expression decreases in cells or tissues subjected to increased temperature (2). The CIRBP protein is composed of an amino-terminal RNA-binding domain and a carboxyl-terminal, glycine-rich domain (1). Stressful stimuli, such as hypoxia, heat shock, osmotic shock, or oxidative conditions, lead to translocation of CIRBP from the nucleus to cytoplasmic stress granules through a mechanism involving CIRBP methylation-dependent nuclear export (3). CIRBP plays a role in regulating apoptosis and preserving the stemness of neural stem cells at moderately low temperatures (4). Research studies demonstrate that CIRBP contributes to the regulation of circadian rhythm through post-translational modulation of CLOCK expression (5).

Database Links

UniProt ID: Q14011

Entrez-Gene Id: 1153

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.

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CIRBP Antibody #13713