CISD1/mitoNEET (D5M4C) Rabbit mAb #83775
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 12 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Protocol
Specificity / Sensitivity
CISD1/mitoNEET (D5M4C) Rabbit mAb recognizes endogenous levels of total CISD1/mitoNEET protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human CISD1/mitoNEET protein.
Background
CDGSH iron-sulfur domain-containing protein 1 (CISD1), also known as mitochondrial outer membrane iron-sulfur (2Fe-2S) protein (mitoNEET) was first identified as the target for the drug pioglitazone, used to treat diabetes (1-3). CISD1/mitoNEET regulates both mitochondrial iron transport into the matrix and mitochondrial respiratory capacity. It has also been shown to affect the dynamics of cellular and whole-body lipid homeostasis (2,4). Furthermore, research studies have shown that CISD1/mitoNEET is overexpressed in human epithelial breast cancer cells and it has been considered a potential chemotherapeutic target (6).
Limited Uses
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