Render Target: STATIC
Render Timestamp: 2025-01-22T13:51:58.718Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:54:04.578
Product last modified at: 2025-01-09T11:45:18.539Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Citrate Synthase (D7V8B) Rabbit mAb #14309

Filter:
  • WB
  • IF
  • F
Western Blotting Image 1: Citrate Synthase (D7V8B) Rabbit mAb
Western blot analysis of extracts from various cell lines using Citrate Synthase (D7V8B) Rabbit mAb.

To Purchase # 14309

Cat. # Size Qty. Price Ships
14309T 20 µl
$167
14309S 100 µl
$339

Supporting Data

REACTIVITY H M R Hm Mk
SENSITIVITY Endogenous
MW (kDa) 45
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Hm-Hamster 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Immunocytochemistry) 1:50 - 1:100
Flow Cytometry (Fixed/Permeabilized) 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Citrate Synthase (D7V8B) Rabbit mAb recognizes endogenous levels of total citrate synthase protein.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human citrate synthase protein.

Background

Citrate synthase (CS) is a mitochondrial enzyme that catalyzes the first and rate-limiting reaction of the citric acid cycle (1,2). The enzyme is responsible for the conversion of oxaloacetate and acetyl-CoA to citrate and CoA (1,2). Research studies show that CS knockdown HeLa cells are more malignant than vector-transfected cells from an in vivo xenograft model, which suggests a direct link between the Warburg Effect and tumor growth (2). Intervertebral disc degeneration, a growing health problem, has been linked to changes in citrate synthase activity (3). 
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.