Claudin-6 (E7U2O) XP® Rabbit mAb #18932
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 23 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:200 - 1:800 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Storage
For a carrier free (BSA and azide free) version of this product see product #59648.
Protocol
Specificity / Sensitivity
Claudin-6 (E7U2O) XP® Rabbit mAb recognizes endogenous levels of total Claudin-6 protein. This antibody does not cross-react with Claudin-9 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val220 of human Claudin-6 protein.
Background
Tight junctions, or zonula occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed primarily in keratinocytes (4) and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines (5,6).
Claudin-6 is a member of the CLDN family that is expressed in epithelial cell sheets. Downregulation of Claudin-6 has been reported in breast invasive ductal carcinoma associated with lymphatic metastasis which may point to a function of Claudin-6 as a tumor suppressor. Claudin-6 is reported to play a role in inhibiting malignancy of breast cancer cells by inducing apoptosis, inhibiting proliferation and migration. Mechanisms of action of Claudin-6 have been described through various signaling pathways such as p38-MAPK, JAKs-STATs, ASK1-JNK, and other pathways (7,8). Regulation of Claudin-6 expression may occur through epigenetic mechanisms (9). Other reports describe aberrant expression in various malignancies (10,11). The clinical significance of Claudin-6 dysregulation has created interest in the potential for pharmaceutical intervention (12-14).
- Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
- Oliveira, S.S. and Morgado-Díaz, J.A. (2007) Cell Mol Life Sci 64, 17-28.
- Hewitt, K.J. et al. (2006) BMC Cancer 6, 186.
- Brandner, J.M. et al. (2002) Eur J Cell Biol 81, 253-63.
- Krämer, F. et al. (2000) Hum Genet 107, 249-56.
- Swisshelm, K. et al. (1999) Gene 226, 285-95.
- Wu, Q. et al. (2013) Chin Med J (Engl) 126, 3539-44.
- Guo, Y. et al. (2016) Int J Oncol 48, 2435-44.
- Liu, Y. et al. (2016) J Exp Clin Cancer Res 35, 120.
- Zhang, X. et al. (2015) Med Oncol 32, 148.
- Torres-Martínez, A.C. et al. (2017) Exp Cell Res 350, 226-235.
- Micke, P. et al. (2014) Int J Cancer 135, 2206-14.
- Ben-David, U. et al. (2013) Nat Commun 4, 1992.
- Schneider, I.C. et al. (2018) Biotechnol J 13, e1700345.
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