Name: Cleaved Caspase-6 (Asp179) (E8L9T) Rabbit mAb
Price: 423.00 USD
Availability: InStock

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WB

Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	18
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Cleaved Caspase-6 (Asp179) (E8L9T) Rabbit mAb recognizes endogenous levels of caspase-6 protein only when cleaved at Asp179. Non-specific bands were observed upon apoptosis at 25, 40, and 200 kDa in some cell lines.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp179 of human caspase-6 protein.

Background

Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes (1,2). Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated (3). The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis (4,5). One of the major targets for caspase-6 is the membrane associated protein lamin A (6). The cleavage of this protein causes cell membrane malfunction, membrane blebbing, and eventual cell death.

Database Links

UniProt ID: P55212

Entrez-Gene Id: 839

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cleaved Caspase-6 (Asp179) (E8L9T) Rabbit mAb #16046