Render Target: STATIC
Render Timestamp: 2025-01-21T12:50:29.869Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:56:49.469
Product last modified at: 2025-01-01T09:01:33.557Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Cleaved Caspase Substrate Motif [DE(T/S/A)D] MultiMab® Rabbit mAb mix #8698

Filter:
  • WB
Western Blotting Image 1: Cleaved Caspase Substrate Motif [DE(T/S/A)D] MultiMab®  Rabbit mAb mix
Western blot analysis of NIH/3T3 and HeLa cells, untreated (-) or treated with Staurosporine #9953 (1 μM, 3 hr; +), using Cleaved Caspase Substrate Motif [DE(T/S/A)D] MultiMab® Rabbit mAb mix (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 as a loading control (lower).

To Purchase # 8698

Cat. # Size Qty. Price Ships
8698T 20 µl
$187
8698S 100 µl
$431

Supporting Data

REACTIVITY All
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • All-All Species Expected 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Cleaved Caspase Substrate Motif [DE(T/S/A)D] MultiMab® Rabbit mAb mix recognizes endogenous levels of caspase-cleaved proteins with a carboxy-terminal aspartic acid residue, and in rare cases a carboxy-terminal glutamic acid residue . This antibody does not cross-react with whole proteins or those ending with a different carboxy-terminal residue.

Species Reactivity:

All Species Expected

Source / Purification

MultiMab® rabbit monoclonal mix antibodies are prepared by combining individual rabbit monoclonal clones in optimized ratios for the approved applications. Each antibody in the mix is carefully selected based on motif recognition and performance in multiple assays. Each mix is engineered to yield the broadest possible coverage of the modification being studied while ensuring a high degree of specificity for the modification or motif.

Background

Apoptosis is a physiological process resulting in a highly regulated, programmed form of cell death that is a normal part of growth and development in multicellular organisms. Caspases are aspartic acid-directed cysteine proteases that are central to the apoptotic mechanism (1). The intrinsic pathway initiates an apoptotic cascade from signals originating within the cell, such as DNA damage, while an extrinsic pathway initiates apoptosis in response to extracellular signals, like FasL. In both intrinsic and extrinsic pathways, initiator caspases cleave downstream substrates, including multiple effector caspases and the primary executioner of cell death, caspase-3 (2,3). Effector caspases amplify the apoptotic cascade to target many critical proteins needed for normal cell function. Apart from its role in developmental biology, the regulation of apoptosis has broad implications for the study of cancer, autoimmune disorders and infectious diseases (4). Thousands of known and putative caspase cleavage sites are present within the human proteome; almost all sites involve cleavage at an aspartic acid residue, though cleavage at glutamic acid residues is seen, rarely, as well (5).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
MultiMab is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.