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Render Timestamp: 2024-12-26T11:23:48.798Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:31:54.837
Product last modified at: 2024-12-23T13:15:16.941Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. Cleaved Drosophila Dcp-1 (Asp215) Antibody

Cleaved Drosophila Dcp-1 (Asp215) Antibody #9578

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY Dm
    SENSITIVITY Endogenous
    MW (kDa) 22
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • Dm-D. melanogaster 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cleaved Drosophila Dcp-1 (Asp215) Antibody recognizes endogenous levels of the large 22 kDa fragment of cleaved Dcp-1. This antibody does not recognize full length Dcp-1. The antibody also detects a non-specific, apoptotic-related band at 50 kDa by western blot.

    Species Reactivity:

    D. melanogaster

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp215 of Drosophila Dcp-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Cell death in the fruit fly Drosophila melanogaster is regulated by many of the same stimuli as mammalian cell death (1). The Drosophila genome contains seven caspase genes; three encode initiator caspases, and four encode effector caspases (reviewed in (2)). The Drosophila effector caspase, death caspase-1 (Dcp-1), is a critical executioner of apoptosis. It is involved in the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). The activation of Dcp-1 requires proteolytic processing of its inactive zymogen into active p22 and p13 fragments (3). Comparison of the in vivo activity between DrICE and Dcp-1 has shown that DrICE is a more effective inducer of apoptosis than Dcp-1, which instead plays a role in determining the rate of cell death (4).

    Database Links

    UniProt ID: O02002


    Entrez-Gene Id: 37729


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