Render Target: STATIC
Render Timestamp: 2024-12-20T11:50:26.450Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-04-05 20:42:58.763
Product last modified at: 2024-12-17T19:04:53.285Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Cleaved PARP (Asp214) (19F4) Mouse mAb

Cleaved PARP (Asp214) (19F4) Mouse mAb #9546

Filter:
  • WB

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 89
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:2000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific) detects endogenous levels of the large fragment (89 kDa) of PARP1 resulting from cleavage at aspartic acid 214. This antibody will detect high levels of full length PARP.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic PARP peptide corresponding to carboxy-terminal residues surrounding Asp214 in human PARP.

    Background

    PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

    (This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)

    Database Links

    UniProt ID: P09874


    Entrez-Gene Id: 142


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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