Name: Cleaved-PARP (Asp214) (E2T4K) Mouse mAb
Price: 383.00 USD
Availability: InStock

Filter:

WB
IP
IHC
IF
F

Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	89
Source/Isotype	Mouse IgG1

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IHC-Immunohistochemistry
IF-Immunofluorescence
F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Immunohistochemistry (Paraffin)	1:50
Immunofluorescence (Immunocytochemistry)	1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized)	1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Cleaved PARP (Asp214) (E2T4K) Mouse mAb recognizes endogenous levels of the large fragment (89 kDa) of human PARP1 protein produced by caspase cleavage. This antibody does not recognize full-length PARP1 or other PARP isoforms.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp214 of human PARP protein.

Background

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

Database Links

UniProt ID: P09874

Entrez-Gene Id: 142

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Cleaved-PARP (Asp214) (E2T4K) Mouse mAb #32563