Render Target: STATIC
Render Timestamp:
4/4/2025, 7:46:53 AM EDT
4/4/2025, 11:46:53 AM UTC
Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-04-01 22:06:27.192
Product last modified at: 2025-04-03T08:00:16.957Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CLEC-1 (F2H2R) Rabbit mAb #80402

Filter:
  • WB
  • IP
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 30-50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200
    Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:400
    Flow Cytometry (Live) 1:200 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CLEC-1 (F2H2R) Rabbit mAb recognizes endogenous levels of total CLEC-1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CLEC-1 protein.

    Background

    C-type lectin domain family 1 member A (CLEC-1) acts as a pattern recognition receptor for pathogens as a member of the C-type lectin superfamily (1). This protein family plays an important role in innate immunity and the adaptive immune response by stimulating cytokine secretion or T cell priming (1,2). CLEC-1 mRNA is primarily expressed in human endothelial and dendritic cells (DCs) and can be upregulated after stimulation with TGF-β (2,3). CLEC-1 localizes to the endoplasmic reticulum membrane fraction, showing a primarily intracellular localization and function (2,3). CLEC-1 may represent a target for cancer immunotherapy, as it has been shown to act as an immune checkpoint in myeloid cells (4). Absence of CLEC-1 reduced immunosuppressive myeloid cells in tumors and invigorated the activation state of DCs, thereby increasing T cell responses (4). Tripartite Motif Containing 21 (TRIM21) has been identified as a ligand for CLEC-1 (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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