Render Target: STATIC
Render Timestamp: 2024-12-26T11:54:15.893Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:07.039
Product last modified at: 2024-12-09T12:45:19.379Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

COL2A1 (E8S2S) Rabbit mAb #43306

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 200
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    COL2A1 (E8S2S) Rabbit mAb recognizes endogenous levels of total COL2A1 protein. This antibody detects a non-specific 62 kDa band with unknown origin.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala194 of human COL2A1 protein.

    Background

    COL2A1 (α1 type II collagen) is a fibril-forming collagen encoded by the gene COL2A1 (1,2). The protein forms homotrimeric type II collagen and is the main extracellular component in hyaline cartilage extracellular matrix (ECM), nucleus pulposus of intervertebral discus, vitreous humor of the eye, and inner ear structure (3). Type II collagen plays a fundamental role in maintaining cartilage formation, differentiation, and growth (4-6). Mutations of COL2A1 cause impairment of ECM deposition and signaling, which contribute to various forms of tissue dysplasia and pathologies such as Stickler syndrome and chondrosarcoma (3,7,8). At the ECM, COL2A1 interacts with integrins such as integrin β1 and growth factors such as IL1β, IL6, IL8, and others, which activate intracellular signaling pathways, including SMAD1, and MAP kinases p38, JNK, and ERK (9-12).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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