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Render Timestamp: 2024-11-21T14:08:33.394Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-20 06:22:29.725
Product last modified at: 2024-10-23T14:15:10.556Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. COL5A1 Antibody

COL5A1 Antibody #37304

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 250
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    COL5A1 Antibody recognizes endogenous levels of total COL5A1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro364 of human COL5A1 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    The Extracellular Matrix (ECM) is a complex network of macromolecules that provides structural tissue support to cells in the basement membrane and interstitial matrix. It is composed of many molecules including proteins, glycoproteins, proteoglycans, and polysaccharides (1,2). One of the major proteins that comprises the ECM, and the human body, is collagen. Collagens are a large family of proteins. They are trimeric molecules comprised of three alpha polypeptide chains that form a triple helix structure that is characteristic of all collagens (3). The large family of collagens is divided into three subgroups: the fibrillar collagens, the non-fibril forming collagens, and the fibril-associated collagens. These sub groups differ in their structure and supramolecular assembly (3). COL5A1 is a minor fibrillar collagen expressed broadly across tissues. It participates in bone and cartilage development, and defects in the COL5A1 gene leads to Ehlers-Danlos Syndrome, a condition that affects the integrity of bones, cartilage, and skin (4,5). Several proteomics, gene profiling, and biochemical studies have indicated that aberrant COL5A1 may play a malignant role in various types of cancer including breast, renal, colon, prostate, lung, and others (6-10).
    COL5A1 is a minor fibrillar collagen expressed broadly across tissues. It participates in bone and cartilage development, and defects in the COL5A1 gene leads to Ehlers-Danlos Syndrome, a condition that affects the integrity of bones, cartilage and skin (4,5). Several proteomics, gene profiling, and biochemical studies have indicated that aberrant COL5A1 may play a malignant role in various types of cancer including breast, renal, colon, prostate, lung, and others (6-11).
    1. Barkan, D. et al. (2010) Eur J Cancer 46, 1181-8.
    2. Hynes, R.O. (2009) Science 326, 1216-9.
    3. Ricard-Blum, S. (2011) Cold Spring Harb Perspect Biol 3, a004978.
    4. Wu, Y.F. et al. (2010) Matrix Biol 29, 701-6.
    5. Kuroda, Y. et al. (2018) Congenit Anom (Kyoto) 58, 191-193.
    6. Wang, S. et al. (2019) Theranostics 9, 4342-4353.
    7. Feng, G. et al. (2019) Cancer Manag Res 11, 1263-1274.
    8. Wu, M. et al. (2019) Oncol Rep 42, 151-175.
    9. Chen, H.C. et al. (2019) J Oral Pathol Med 48, 468-476.
    10. Nguyen, E.V. et al. (2019) Mol Cell Proteomics 18, 1410-1427.
    11. Wu, Y.F. et al. (2010) Matrix Biol 29, 701-6.
    12. Kuroda, Y. et al. (2018) Congenit Anom (Kyoto) 58, 191-93.
    13. Wang, S. et al. (2019) Theranostics 9, 4342-53.
    14. Feng, G. et al. (2019) Cancer Manag Res 11, 1263-74.
    15. Wu, M. et al. (2019) Oncol Rep 42, 151-75.
    16. Chen, H.C. et al. (2019) J Oral Pathol Med 48, 468-76.
    17. Nguyen, E.V. et al. (2019) Mol Cell Proteomics 18, 1410-27.

    Database Links

    UniProt ID: P20908


    Entrez-Gene Id: 1289


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