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Render Timestamp:
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4/4/2025, 7:57:00 PM UTC
Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-07 13:18:46.201
Product last modified at: 2025-01-24T23:30:10.816Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

COL6A1 (E9Q3P) Rabbit mAb #58232

Filter:
  • WB
  • IF
Western Blotting Image 1: COL6A1 (E9Q3P) Rabbit mAb
Western blot analysis of extracts from various cell lines using COL6A1 (E9Q3P) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of COL6A1 protein in HCT-15 cells is consistent with the predicted expression pattern.

To Purchase # 58232

Cat. # Size Qty. Price
58232T 20 µl
$168
58232S 100 µl
$371

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 130
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:50 - 1:250
Immunofluorescence (Frozen) 1:3200 - 1:6400
Immunofluorescence (Immunocytochemistry) 1:3200 - 1:12800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

COL6A1 (E9Q3P) Rabbit mAb recognizes endogenous levels of total COL6A1 protein. Non-specific cytoplasmic labeling of Purkinje cells in the cerebellum, acinar cells in pancreas, and tubules in testis may be observed by immunofluorescence.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile536 of human COL6A1 protein.

Background

Collagen type VI (ColVI) is a microfibrillar collagen found in the extracellular matrix (ECM) of muscles, bone, and connective tissues (1). ColVI consists of three main alpha chains: alpha 1, alpha 2, and alpha 3, which are encoded by the COL6A1, COL6A2, and COL6A3 genes, respectively. The three alpha chains form triple helix monomers and tetramers, which further assemble into a beaded microfilament network structure in the ECM (1,2). ColVI interacts with other ECM components, such as collagens, fibronectins, and perlecan, to support ECM mechanical sensing, anchoring the basement membrane to the surrounding ECM and inhibiting apoptosis and oxidative damage (3,4). Mutations in each of the ColVI genes (COL6A1, COL6A2, and COL6A3) result in defective ColVI assembly, causing Ullrich congenital muscular dystrophy (CMD) and Bethlem myopathy due to malformation of ECM structure (5,6). Knockout of COL6A1 in mice displays a mild myopathy and neurodegeneration associated with mitochondrial dysfunction, defective autophagy, and spontaneous apoptosis of muscle fibers (7,8). Increased COL6A1 in the ECM promotes tumor growth, metastasis, and therapeutic drug resistance (9-11).
For Research Use Only. Not For Use In Diagnostic Procedures.
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