Render Target: STATIC
Render Timestamp: 2025-01-21T12:46:01.865Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:54:47.953
Product last modified at: 2025-01-01T09:04:33.596Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Complexin-1/2 (D8A6E) Rabbit mAb #28070

Filter:
  • WB
  • IP
  • IF
Western Blotting Image 1: Complexin-1/2 (D8A6E) Rabbit mAb
Western blot analysis of extracts from Neuro-2a, NIH/3T3, and mouse brain, using Complexin-1/2 (D8A6E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 28070

Cat. # Size Qty. Price Ships
28070T 20 µl
$153
28070S 100 µl
$339

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 14-16
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunofluorescence (Immunocytochemistry) 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Complexin-1/2 (D8A6E) Rabbit mAb recognizes endogenous levels of total complexin-1 and complexin-2 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro125 of human complexin-2 protein.

Background

Complexins are small soluble proteins composed of a central α-helical-structured domain surrounded by amino- and carboxy-terminal unstructured domains (1). These cytosolic proteins bind to t-SNAREs with low affinity and to assembled SNARE complexes with high affinity (1,2). Two isoforms, complexin-1 and complexin-2, are expressed in neuronal cells (3) where they regulate evoked and spontaneous exocytosis (4,5). Altered complexin expression resulting from RNAi-mediated knockdown (6) or gene invalidation (7) leads to alteration in spontaneous fusion events and neurotransmitter release, which reflects functions at both inhibitory and stimulatory synapses.
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.