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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Cool1/βPix Antibody #4515

Filter:
  • WB
  • IP
Western Blotting Image 1: Cool1/βPix Antibody
Western blot analysis of extracts from Jurkat, NIH/3T3, ZR-75, MCF-7 and COS cells using Cool1/βPix Antibody.

To Purchase # 4515

Cat. # Size Qty. Price Ships
4515S 100 µl
$306

Supporting Data

REACTIVITY H M R Hm Mk
SENSITIVITY Endogenous
MW (kDa) 81
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Hm-Hamster 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Cool1/βPix Antibody detects endogenous levels of total cool1/βPix protein. The antibody does not cross-react with cool2/αPix.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues in the center of human cool1/βPix. Antibodies are purified by peptide affinity chromatography.

Background

Cool/Pix proteins comprise a family of guanine nucleotide exchange factors (GEFs) localized to focal adhesions. The family consists of two isoforms, cool2/αpix and cool1/βPix, the latter having two splice variants that vary in their carboxy termini (1). Cool1/βPix, like other GEFs, has a DH (Dbl homology) domain, which allows binding of small GTPases and GDP/GTP exchange, and a PH (Pleckstrin homology) domain, which is important in regulating subcellular localization. Cool1/βPix also has an SH3 domain, which binds to the PAK kinase, a downstream effector of cdc42 and Rac (3,4). Phosphorylation of cool1/βPix by PAK2 downstream of MAPK signaling alters the localization of a complex containing PAK2 and cool-1/βPix, regulating formation of growth cones in response to growth factors (4). Growth factor induced activation of Rac1 via cool1/βPix was later shown to occur independently of subcellular localization (5). Endothelin-1 stimulation of mesangial cells stimulates the protein kinase A (PKA) pathway, resulting in translocation of cool-1/βPix and activation of cdc42 (6).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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