COX2/MT-CO2 (E6U9K) Rabbit mAb #50003

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Supporting Data

REACTIVITY	H Mk
SENSITIVITY	Endogenous
MW (kDa)	19
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

COX2/MT-CO2 (E6U9K) Rabbit mAb recognizes endogenous levels of total COX2/MT-CO2 protein.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly222 of human COX2/MT-CO2 protein.

Background

The electron transport chain (or respiratory chain) is a series of mitochondrial protein complexes (Complexes I-IV) that utilize coupled reduction-oxidation (redox) reactions to transfer electrons from donor molecules to acceptors. By coupling this electron transport with active proton pumping across the inner mitochondrial membrane, an electrochemical gradient is generated that is used to drive ATP synthesis (1). Complex IV (cytochrome c oxidase) is the terminal complex of the respiratory chain, responsible for transporting electrons from cytochrome c to molecular oxygen. COX2 (cytochrome c oxidase subunit 2), also known as MT-CO2, is one of 14 subunits of cytochrome c oxidase, and one of three subunits (COX1/MT-CO1, COX2/MT-CO2, and COX3/MT-CO3) encoded by the mitochondrial genome (2). These three mitochondrial-encoded subunits form the functional core of cytochrome c oxidase. Research studies have shown that disruptions to COX2/MT-CO2 function can have deleterious health effects. For example, a novel point mutation in the COX2/MT-CO2 gene was identified as a risk factor for cardiovascular disease (3), whereas a separate point mutation was causally linked to adult onset cerebellar ataxia (4).

Database Links

UniProt ID: P00403

Entrez-Gene Id: 4513

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For Research Use Only. Not For Use In Diagnostic Procedures.

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